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Pakhtakor Tashkent ill participate at the tournament as Uzbekistan champions, while Bunyodkor book tickets to tournament after triumph in Uzbekistan Cup.
Locomotiv Tashkent, which claimed the third place in Uzbekistan league, will also play in the AFC Champions League.
Pakhtakor and Bunyodkor will kick off their campaign from group stage of the league, while Locomotiv will play in qualification round.