Pakhtakor, who are leading Group A with 13 points with a game remaining, could seal a home match in the round of 16 with a draw against Saudi Arabia’s Al Hilal on May 20.
Bunyodkor, who needs to avoid defeat in their final continental game against UAE’s Al Shabab Al Arabi at home on May 19 to qualify for the last 16, brushed aside Bukhara 4-0 to lead the 16-team standings with 27 points, four more than Pakhtakor.
Darko Markovic scored twice for the ACL ever-presents while Stanislav Andreev, Dilshod Sharofetdinov and Zaynitdin Tadjiev added a goal each.
A brace from Anvar Soliev a goal apiece from Jose Villanueva and Edson Ramos saw Bunyodkor maintain their perfect record at home.
In other matches, Metallurg were held 1-1 by Locomotive, Sogdiana beat Navabhor 1-0, Dynamo played a 0-0 draw with Nasaf, Mashal lost to Shurtan 2-1, Khorezm drew 1-1 with Almalik and AFC Cup last 16 hopefuls Neftchi beat Andijan 2-0.
STANDINGS: 1. Bunyodkor 27, 2. Pakhtakor 23, 3. Nasaf 20, 4. Almalik 17, 5. Locomotive 15.